Protocols and Data Download

Here you can download some of the protocols our lab is using, as well as some unpublished data.

Protocols

- ChIP protocol (optimized for few cells, <100.000 per IP)

- Mapping open chromatin with FAIRE

- Human islet culture and processing for RNA and chromatin studies

- EMSA assay

- Concerning Nat Genet. 2010 Mar;42(3):255-9. Epub 2010 Jan 31. :

BED file (as output of MACS peak calling algorithm) of FAIRE open chromatin sites consistent through different human islets biological replicates samples.

- Concerning Genome Res. 2010 Jun;20(6):722-32. Epub 2010 Apr 15. :

COD files as output by Cisgenome HMM analysis on Affymetrix mouse promoter arrays. These files contain the enriched intervals (mm8/NCBI36) for H3K4me3 and H3K27me3 for all analyzed cell types: part1, part2.